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KMID : 0380020170320010035
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Korean Journal of Biotechnology and Bioengineering
2017 Volume.32 No. 1 p.35 ~ p.45
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Development of Optical Fiber Glucose and Lactate Biosensors for Bioprocess Monitoring
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Jung Chang-Hwan
Sohn Ok-Jae
Rhee Jong-Il
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Abstract
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In this work the optical fiber glucose and lactate biosensors were developed by using fluorescent dye and enzyme immobilized on the end tip of an optical fiber. 3-Glycidyloxypropyl) methyldiethoxysilane (GPTMS), (3-Aminopropyl) trimethoxysilane (APTMS) and Methyltrimethoxysilane (MTMS) were used to immobilize glucose oxidase (GOD), lactate oxidase (LOD) and ruthenium(II) complex (tris(4,7-diphenyl-1,10-phenanthroline) ruthenium(II), Ru(dpp)32+) as oxygen sensitive fluorescent dye. MTMS sol-gel was an excellent supporting material for the immobilization of Ru(dpp)32+, GOD, and LOD on the optical fiber. Storage stability of the optical fiber glucose sensor was kept constant over 20 days, while the optical fiber lactate sensor had constant storage stability over 17 days. The optical fiber glucose and lactate biosensors also maintained good operational stability for 20 hours and 14 hours, respectively. The activities of the immobilized enzymes were most excellent at pH 7 and at 25oC. On-line monitoring of glucose and lactate in a simulated process was performed with the optical fiber glucose and lactate biosensors. On-line monitoring results were agreed with those of off-line data measured with high performance liquid chromatography (HPLC).
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KEYWORD
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Optical fiber biosensor, Glucose, Lactate, Fluorescent dye, On-line monitoring, Enzyme immobilization
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